Difference between revisions of "Part:BBa K4361113"

Latest revision as of 13:42, 12 October 2022

Blc operator SDM primer R

A site-directed mutagenesis primer, to be used in conjunction with the mutation-introducingforward primer Part:BBa_K4361112. Primers can be used in a PCR reaction with the Part:BBa_K4361115 part as DNA template. A deletion will be introduced in the blc operator sequence, resulting in the altered sequence as described in Part:BBa_K4361114.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 21
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 21
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 21
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 21
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 21
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4361113 short</partinfo>
-A site-directed mutagenesis primer, to be used in conjunction with the mutation-inducing forward primer [[Part:BBa_K4361112]]. When used as primers during the PCR of pSB1C3 containing the Blc operator sequence as an insert ([[Part:BBa_K4361115]]), a deletion will be induced in the operator, resulting in the altered sequence as described in [[Part:BBa_K4361114]]. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence ([[Part:BBa_K4361114]]) may act as a negative control when testing the binding properties of BlcR.
+A site-directed mutagenesis primer, to be used in conjunction with the mutation-introducingforward primer [[Part:BBa_K4361112]]. Primers can be used in a PCR reaction with the [[Part:BBa_K4361115]] part as DNA template. A deletion will be introduced in the <i>blc</i> operator sequence, resulting in the altered sequence as described in [[Part:BBa_K4361114]].
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