Difference between revisions of "Part:BBa K4361113"

 
 
(5 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4361113 short</partinfo>
 
<partinfo>BBa_K4361113 short</partinfo>
  
TBD
+
A site-directed mutagenesis primer, to be used in conjunction with the mutation-introducingforward primer [[Part:BBa_K4361112]]. Primers can be used in a PCR reaction with the [[Part:BBa_K4361115]] part as DNA template. A deletion will be introduced in the <i>blc</i> operator sequence, resulting in the altered sequence as described in [[Part:BBa_K4361114]].
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 9: Line 10:
  
 
<!-- -->
 
<!-- -->
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K4361113 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4361113 SequenceAndFeatures</partinfo>
  

Latest revision as of 13:42, 12 October 2022


Blc operator SDM primer R

A site-directed mutagenesis primer, to be used in conjunction with the mutation-introducingforward primer Part:BBa_K4361112. Primers can be used in a PCR reaction with the Part:BBa_K4361115 part as DNA template. A deletion will be introduced in the blc operator sequence, resulting in the altered sequence as described in Part:BBa_K4361114.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 21
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 21
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 21
  • 1000
    COMPATIBLE WITH RFC[1000]