Difference between revisions of "Part:BBa K4361112"

Latest revision as of 13:38, 12 October 2022

Blc operator SDM primer F

A site-directed mutagenesis primer, to be used in conjunction with the reverse primer Part:BBa_K4361113. Primers can be used in a PCR reaction with the Part:BBa_K4361115 part as DNA template. A deletion will be introduced in the blc operator sequence, resulting in the altered sequence as described in Part:BBa_K4361114.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4361112 short</partinfo>
-A site-directed mutagenesis primer, to be used in conjunction with the reverse primer [[Part:BBa_K4361113]]. When used as primers during the PCR of pSB1C3 containing the Blc operator sequence as an insert ([[Part:BBa_K4361115]]), a deletion will be induced in the operator, resulting in the altered sequence as described in [[Part:BBa_K4361114]]. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence may act as a negative control when testing the binding properties of BlcR.
+A site-directed mutagenesis primer, to be used in conjunction with the reverse primer [[Part:BBa_K4361113]]. Primers can be used in a PCR reaction with the [[Part:BBa_K4361115]] part as DNA template. A deletion will be introduced in the <i> blc </i> operator sequence, resulting in the altered sequence as described in [[Part:BBa_K4361114]].
-A site-directed mutagenesis primer, used to induce the deletion of adenine 20 in the Blc operator sequence ([[Part:BBa_K4361111]]) during PCR, in conjunction with the reverse primer [[Part:BBa_K4361113]]. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence ([[Part:BBa_K4361114]]) may act as a negative control when testing the binding properties of BlcR.
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