Difference between revisions of "Part:BBa K4361112"
 
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<partinfo>BBa_K4361112 short</partinfo>
 
<partinfo>BBa_K4361112 short</partinfo>
  
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A site-directed mutagenesis primer, used to induce the deletion of adenine 20 in the Blc operator sequence ([[Part:BBa_K4361111]]) during PCR, in conjunction with the reverse primer [[Part:BBa_K4361113]]. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence ([[Part:BBa_K4361114]]) may act as a negative control when testing the binding properties of BlcR.
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A site-directed mutagenesis primer, to be used in conjunction with the reverse primer [[Part:BBa_K4361113]]. Primers can be used in a PCR reaction with the [[Part:BBa_K4361115]] part as DNA template. A deletion will be introduced in the <i> blc </i> operator sequence, resulting in the altered sequence as described in [[Part:BBa_K4361114]].  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:38, 12 October 2022


Blc operator SDM primer F

A site-directed mutagenesis primer, to be used in conjunction with the reverse primer Part:BBa_K4361113. Primers can be used in a PCR reaction with the Part:BBa_K4361115 part as DNA template. A deletion will be introduced in the blc operator sequence, resulting in the altered sequence as described in Part:BBa_K4361114.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]