Difference between revisions of "Part:BBa K4361112"

Latest revision as of 13:38, 12 October 2022

Blc operator SDM primer F

A site-directed mutagenesis primer, to be used in conjunction with the reverse primer Part:BBa_K4361113. Primers can be used in a PCR reaction with the Part:BBa_K4361115 part as DNA template. A deletion will be introduced in the blc operator sequence, resulting in the altered sequence as described in Part:BBa_K4361114.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Unknown
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Unknown
23
INCOMPATIBLE WITH RFC[23]
Unknown
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4361112 short</partinfo>
-A site-directed mutagenesis primer, used to induce the deletion of adenine 20 in the Blc operator sequence ([[Part:BBa_K4361111]]) during PCR, in conjunction with the reverse primer [[Part:BBa_K4361113]]. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence ([[Part:BBa_K4361114]]) may act as a negative control when testing the binding properties of BlcR.
+A site-directed mutagenesis primer, to be used in conjunction with the reverse primer [[Part:BBa_K4361113]]. Primers can be used in a PCR reaction with the [[Part:BBa_K4361115]] part as DNA template. A deletion will be introduced in the <i> blc </i> operator sequence, resulting in the altered sequence as described in [[Part:BBa_K4361114]].
 <!-- Add more about the biology of this part here
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-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'><h3>Sequence and Features</h3></span>
 <partinfo>BBa_K4361112 SequenceAndFeatures</partinfo>