Difference between revisions of "Part:BBa K4361112"

 
 
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<partinfo>BBa_K4361112 short</partinfo>
 
<partinfo>BBa_K4361112 short</partinfo>
  
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TBD
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A site-directed mutagenesis primer, to be used in conjunction with the reverse primer [[Part:BBa_K4361113]]. Primers can be used in a PCR reaction with the [[Part:BBa_K4361115]] part as DNA template. A deletion will be introduced in the <i> blc </i> operator sequence, resulting in the altered sequence as described in [[Part:BBa_K4361114]].
  
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Largely overlaps with BBa_K4361111, but with a deletion of the adenine in position 20 to disrupt BlcR binding.
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K4361112 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4361112 SequenceAndFeatures</partinfo>
  

Latest revision as of 13:38, 12 October 2022


Blc operator SDM primer F

A site-directed mutagenesis primer, to be used in conjunction with the reverse primer Part:BBa_K4361113. Primers can be used in a PCR reaction with the Part:BBa_K4361115 part as DNA template. A deletion will be introduced in the blc operator sequence, resulting in the altered sequence as described in Part:BBa_K4361114.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]