Difference between revisions of "Part:BBa K4438705"

 
 
(2 intermediate revisions by one other user not shown)
Line 1: Line 1:
 +
__NOTOC__
 +
<partinfo>BBa_K4438705 short</partinfo>
  
 +
P4G13_trigger_3_w/oPol is single-stranded DNA having 28 nucleotides. Figure 1B) shows the secondary structure and minimum free energy. At both ends 5’ and 3’, few bases are complementary to P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>). The middle region consists of a full ssDNA sense T7 promoter sequence.
 +
 +
===Usage and Biology===
 +
Figure 2D) Shows the secondary structure of both the parts hybridised at 37° Celsius. The hormone binds with P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>) with high affinity [1] and displaces the P4G13_trigger_3_w/oPol (<partsinfo>BBa_K4438705</partsinfo>).
 +
This part has complete complementarity with part P4G13_Target_3 (<partsinfo>BBa_K4438706</partsinfo>). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers.
 +
All three parts can be used to detect different concentrations of progesterone.
 +
[[File:T--IISER-Tirupati_India--Pg413_3.png]]
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K4438705 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K4438705 parameters</partinfo>
 +
<!-- -->
 +
===References===
 +
Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082.

Latest revision as of 13:32, 12 October 2022

P4G13_trigger_3_w/oPol

P4G13_trigger_3_w/oPol is single-stranded DNA having 28 nucleotides. Figure 1B) shows the secondary structure and minimum free energy. At both ends 5’ and 3’, few bases are complementary to P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>). The middle region consists of a full ssDNA sense T7 promoter sequence.

Usage and Biology

Figure 2D) Shows the secondary structure of both the parts hybridised at 37° Celsius. The hormone binds with P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>) with high affinity [1] and displaces the P4G13_trigger_3_w/oPol (<partsinfo>BBa_K4438705</partsinfo>). This part has complete complementarity with part P4G13_Target_3 (<partsinfo>BBa_K4438706</partsinfo>). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. All three parts can be used to detect different concentrations of progesterone. T--IISER-Tirupati India--Pg413 3.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082.