Difference between revisions of "Part:BBa K4438703"
| Line 2: | Line 2: | ||
<partinfo>BBa_K4438703 short</partinfo> | <partinfo>BBa_K4438703 short</partinfo> | ||
| − | P4G13_trigger_2_w/oPol is single-stranded DNA having 33 nucleotides. Figure 1B) shows the secondary structure and minimum free energy. At both ends 5’ and 3’, few bases are complementary to P4G13_aptamer (BBa_K4438700). The middle region consists of a full ssDNA sense T7 promoter sequence. | + | P4G13_trigger_2_w/oPol is single-stranded DNA having 33 nucleotides. Figure 1B) shows the secondary structure and minimum free energy. At both ends 5’ and 3’, few bases are complementary to P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>). The middle region consists of a full ssDNA sense T7 promoter sequence. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Figure 2D) Shows the secondary structure of both the parts hybridised at 37° Celsius. The hormone binds with P4G13_aptamer (BBa_K4438700) with high affinity [1] and displaces the P4G13_trigger_2_w/oPol (BBa_K4438703). | + | Figure 2D) Shows the secondary structure of both the parts hybridised at 37° Celsius. The hormone binds with P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>) with high affinity [1] and displaces the P4G13_trigger_2_w/oPol (<partsinfo>BBa_K4438703</partsinfo>). |
| − | This part has complete complementarity with part P4G13_Target_2 (BBa_K4438704). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. | + | This part has complete complementarity with part P4G13_Target_2 (<partsinfo>BBa_K4438704</partsinfo>). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. |
All three parts can be used to detect different concentrations of progesterone. | All three parts can be used to detect different concentrations of progesterone. | ||
| − | + | [[File:T--IISER-Tirupati_India--Pg413_2.png]] | |
<!-- --> | <!-- --> | ||
Latest revision as of 13:25, 12 October 2022
P4G13_trigger_2_w/oPol
P4G13_trigger_2_w/oPol is single-stranded DNA having 33 nucleotides. Figure 1B) shows the secondary structure and minimum free energy. At both ends 5’ and 3’, few bases are complementary to P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>). The middle region consists of a full ssDNA sense T7 promoter sequence.
Usage and Biology
Figure 2D) Shows the secondary structure of both the parts hybridised at 37° Celsius. The hormone binds with P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>) with high affinity [1] and displaces the P4G13_trigger_2_w/oPol (<partsinfo>BBa_K4438703</partsinfo>).
This part has complete complementarity with part P4G13_Target_2 (<partsinfo>BBa_K4438704</partsinfo>). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers.
All three parts can be used to detect different concentrations of progesterone.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082