Difference between revisions of "Part:BBa K4438702"
 
Line 6: Line 6:
 
===Usage and Biology===
 
===Usage and Biology===
 
This part along with P4G13_trigger_1_w/oPol (<partsinfo>BBa_K4438701</partsinfo>) and P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>) can be used for sensing different concentrations of progesterone [1].
 
This part along with P4G13_trigger_1_w/oPol (<partsinfo>BBa_K4438701</partsinfo>) and P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>) can be used for sensing different concentrations of progesterone [1].
−
https://parts.igem.org/File:T--IISER-Tirupati_India--Pg413_1.png
+
[[File:T--IISER-Tirupati_India--Pg413_1.png]]
  
  

Latest revision as of 13:22, 12 October 2022

P4G13_Target_1

P4G13_Target_1 (<partsinfo>BBa_K4438702</partsinfo>) is a single-stranded DNA sequence having 81 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 3’ end has few bases complementary to P4G13_trigger_1_w/oPol (<partsinfo>BBa_K4438701</partsinfo>). In the middle complete ssDNA antisense T7 promoter sequence is present. The 5’ end has broccoli cDNA sequence.

Usage and Biology

This part along with P4G13_trigger_1_w/oPol (<partsinfo>BBa_K4438701</partsinfo>) and P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>) can be used for sensing different concentrations of progesterone [1]. T--IISER-Tirupati India--Pg413 1.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082.