Difference between revisions of "Part:BBa K4438701"

 
 
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<partinfo>BBa_K4438701 short</partinfo>
  
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P4G13_trigger_1_w/oPol is single-stranded DNA having 38 nucleotides. Figure 1B) shows the secondary structure and minimum free energy. At the 5’ end, a few bases are complementary to P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>). The middle region consists of a full ssDNA sense T7 promoter sequence and at the 3’ end, there are a few bases of ssDNA sense broccoli sequence.
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===Usage and Biology===
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Figure 2D) Shows the secondary structure of both the parts hybridised at 37° Celsius. The hormone binds with P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>) with high affinity [1] and displaces the P4G13_trigger_1_w/oPol (<partsinfo>BBa_K4438701</partsinfo>).
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This part has complete complementarity with the part P4G13_Target_1 (<partsinfo>BBa_K4438702</partsinfo>). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers.
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All three parts can be used to detect different concentrations of progesterone.
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[[File:T--IISER-Tirupati_India--Pg413_1.png]]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4438701 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4438701 parameters</partinfo>
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===References===
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Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082

Latest revision as of 13:18, 12 October 2022

P4G13_trigger_1_w/oPol

P4G13_trigger_1_w/oPol is single-stranded DNA having 38 nucleotides. Figure 1B) shows the secondary structure and minimum free energy. At the 5’ end, a few bases are complementary to P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>). The middle region consists of a full ssDNA sense T7 promoter sequence and at the 3’ end, there are a few bases of ssDNA sense broccoli sequence.

Usage and Biology

Figure 2D) Shows the secondary structure of both the parts hybridised at 37° Celsius. The hormone binds with P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>) with high affinity [1] and displaces the P4G13_trigger_1_w/oPol (<partsinfo>BBa_K4438701</partsinfo>). This part has complete complementarity with the part P4G13_Target_1 (<partsinfo>BBa_K4438702</partsinfo>). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. All three parts can be used to detect different concentrations of progesterone. T--IISER-Tirupati India--Pg413 1.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082