Difference between revisions of "Part:BBa K4169015:Design"

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The gene sequence of tmd is from The European Nucleotide Archive (ENA). Coding: CDX46666.1.
 
The gene sequence of tmd is from The European Nucleotide Archive (ENA). Coding: CDX46666.1.
  
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The link is here: https://www.ebi.ac.uk/ena/browser/view/CDX46666.
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The link is here: https://www.ebi.ac.uk/ena/browser/view/CDX46666
  
 
And tmd gene is originally in Mesorhizobium sp. ORS 3359.
 
And tmd gene is originally in Mesorhizobium sp. ORS 3359.
  
 
===References===
 
===References===
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Jang MH, Basran J, Scrutton NS, Hille R. The reaction of trimethylamine dehydrogenase with trimethylamine. J Biol Chem. 1999 May 7;274(19):13147-54. doi: 10.1074/jbc.274.19.13147. PMID: 10224069.
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[1] Jang M H, Basran J, Scrutton N S, et al. The reaction of trimethylamine dehydrogenase with trimethylamine[J]. Journal of Biological Chemistry, 1999, 274(19): 13147-13154.
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Loechel C, Basran A, Basran J, Scrutton NS, Hall EA. Using trimethylamine dehydrogenase in an enzyme linked amperometric electrode. Part 1. Wild-type enzyme redox mediation. Analyst. 2003 Feb;128(2):166-72. doi: 10.1039/b211895e. PMID: 12625558.
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Because trimethylamine dehydrogenase (TMADH) and dimethylamine dehydrogenase (DMADH) exist as dimers
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Huang L, Rohlfs RJ, Hille R. The reaction of trimethylamine dehydrogenase with electron transferring flavoprotein. J Biol Chem. 1995 Oct 13;270(41):23958-65. doi: 10.1074/jbc.270.41.23958. PMID: 7592591.
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 +
[2] Scrutton N S, Sutcliffe M J. Trimethylamine dehydrogenase and electron transferring flavoprotein[J]. Enzyme-Catalyzed Electron and Radical Transfer, 2000: 145-181.

Latest revision as of 13:14, 12 October 2022


produce TMADH


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 388
    Illegal PstI site found at 183
    Illegal PstI site found at 1782
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 388
    Illegal PstI site found at 183
    Illegal PstI site found at 1782
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 388
    Illegal XhoI site found at 1717
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 388
    Illegal PstI site found at 183
    Illegal PstI site found at 1782
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 388
    Illegal PstI site found at 183
    Illegal PstI site found at 1782
    Illegal AgeI site found at 879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GenScript synthesised tmd plasmids and performed codon optimization.

We added His tag on the end of tmd sequence.

Source

The gene sequence of tmd is from The European Nucleotide Archive (ENA). Coding: CDX46666.1.

The link is here: https://www.ebi.ac.uk/ena/browser/view/CDX46666

And tmd gene is originally in Mesorhizobium sp. ORS 3359.

References

[1] Jang M H, Basran J, Scrutton N S, et al. The reaction of trimethylamine dehydrogenase with trimethylamine[J]. Journal of Biological Chemistry, 1999, 274(19): 13147-13154. Because trimethylamine dehydrogenase (TMADH) and dimethylamine dehydrogenase (DMADH) exist as dimers

[2] Scrutton N S, Sutcliffe M J. Trimethylamine dehydrogenase and electron transferring flavoprotein[J]. Enzyme-Catalyzed Electron and Radical Transfer, 2000: 145-181.