Difference between revisions of "Part:BBa K4438813"

 
 
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<partinfo>BBa_K4438813 short</partinfo>
  
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MN4_trigger_7_3’trigger_phi29(<partinfo>BBa_K4438813</partinfo>) is single-stranded DNA having 11 nucleotides. Figure 1B) shows the secondary structure and minimum free energy. The sequence is complementary to the 3’ end of the Aptamer_MN4 (<partinfo>BBa_K4438800</partinfo>) with no mismatched bases.
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===Usage and Biology===
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The part MN4_trigger_7_3’trigger_phi29(<partinfo>BBa_K4438813</partinfo>) has complementarity to the part Aptamer_MN4 (<partinfo>BBa_K4438800</partinfo>) and disrupts its three-way junction.
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Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. Quinine binds with cocaine-binding aptamer MN4 [1] and displaces the MN4_trigger_7_3’trigger_phi29(<partinfo>BBa_K4438813</partinfo>). This part has complete complementarity with MN4_Target_7(<partinfo>BBa_K4438814</partinfo>). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers.
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Different levels of quinine can be detected using all these three parts.
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[[File:T--IISER-Tirupati_India--mn4structure7.png]]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4438813 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4438813 parameters</partinfo>
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===References===
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Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.

Latest revision as of 13:14, 12 October 2022

MN4_trigger_7_3trigger_phi29

MN4_trigger_7_3’trigger_phi29(BBa_K4438813) is single-stranded DNA having 11 nucleotides. Figure 1B) shows the secondary structure and minimum free energy. The sequence is complementary to the 3’ end of the Aptamer_MN4 (BBa_K4438800) with no mismatched bases.

Usage and Biology

The part MN4_trigger_7_3’trigger_phi29(BBa_K4438813) has complementarity to the part Aptamer_MN4 (BBa_K4438800) and disrupts its three-way junction. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. Quinine binds with cocaine-binding aptamer MN4 [1] and displaces the MN4_trigger_7_3’trigger_phi29(BBa_K4438813). This part has complete complementarity with MN4_Target_7(BBa_K4438814). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. Different levels of quinine can be detected using all these three parts.

T--IISER-Tirupati India--mn4structure7.png



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.