Difference between revisions of "Part:BBa K4273017"
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[[Image:t-links-china-figure50.png|thumb|left|900px|'''Figure 1: Different combinations of AGL-ALAL genes found from different MAA-producing marine organisms. We used the strongest yeast constitutive promoter pTDH3 to express all AG-L genes, and the strong promoter pPGK1 to express all ALA-L genes, then inserted the 9 different combinations into 2μ plasmid vectors and transformed the plasmids into L3 to obtain L5 strain''']] | [[Image:t-links-china-figure50.png|thumb|left|900px|'''Figure 1: Different combinations of AGL-ALAL genes found from different MAA-producing marine organisms. We used the strongest yeast constitutive promoter pTDH3 to express all AG-L genes, and the strong promoter pPGK1 to express all ALA-L genes, then inserted the 9 different combinations into 2μ plasmid vectors and transformed the plasmids into L3 to obtain L5 strain''']] | ||
Revision as of 13:02, 12 October 2022
pTDH3-DDGS-tTDH1-pPGK1-OMT-tPGK1
The first and second gene of gene clusters involved in biosynthesis of shinorine in cyanobacteria N. punctiforme.
encoding 2-demethyl 4-deoxygadusol synthase (DDGS) that converts sedoheptulose 7-phosphate (S7P) to 2-demethyl-4-deoxygadusol (DDG) and O-methyltransferase (O-MT) that converts 2-demethyl-4-deoxygadusol (DDG) to 4-deoxygadusol (4-DG)
Usage and Biology
Figure 1: Different combinations of AGL-ALAL genes found from different MAA-producing marine organisms. We used the strongest yeast constitutive promoter pTDH3 to express all AG-L genes, and the strong promoter pPGK1 to express all ALA-L genes, then inserted the 9 different combinations into 2μ plasmid vectors and transformed the plasmids into L3 to obtain L5 strain
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3270
Illegal XhoI site found at 1922 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2168
Illegal BsaI.rc site found at 3280