Difference between revisions of "Part:BBa K4129120"
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===Usage and Biology===
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=== Fungal synthetic expression system using FunsTF01 ===
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This composite part was used to characterise the synthetic transcription factor (sTF) FunsTF01. The reporter cassette consists of the synthetic 6xLexO-Pmin (BBa_K4129120) that transcribe mCherry.
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In this fungal synthetic expression system (SES) FunsTF01 was constitutively expressed from P<i>gpdA</i>. The FunsTF01, which contains a LexA DNA-binding domain, would be able to bind to the LexO binding sites for the minimal promoter fused with LexO binding sites (6xLexO-Pmin). The efficiency of the FunsTF01 activation was measured by the reporter gene mCherry, which is expressed from the 6xLexO-Pmin.
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For characterisation look up BBa_K4129100.
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Latest revision as of 12:57, 12 October 2022


The fungal synthetic expression system with FunsTF01 and 6xLexO-Pmin


Fungal synthetic expression system using FunsTF01

In this fungal synthetic expression system (SES) FunsTF01 was constitutively expressed from PgpdA. The FunsTF01, which contains a LexA DNA-binding domain, would be able to bind to the LexO binding sites for the minimal promoter fused with LexO binding sites (6xLexO-Pmin). The efficiency of the FunsTF01 activation was measured by the reporter gene mCherry, which is expressed from the 6xLexO-Pmin.

For characterisation look up BBa_K4129100.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4174
    Illegal XbaI site found at 6087
    Illegal PstI site found at 141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
    Illegal NheI site found at 2986
    Illegal PstI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 145
    Illegal BamHI site found at 2920
    Illegal BamHI site found at 3512
    Illegal BamHI site found at 5368
    Illegal XhoI site found at 701
    Illegal XhoI site found at 1083
    Illegal XhoI site found at 3661
    Illegal XhoI site found at 4038
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4174
    Illegal XbaI site found at 6087
    Illegal PstI site found at 141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4174
    Illegal XbaI site found at 6087
    Illegal PstI site found at 141
    Illegal NgoMIV site found at 3078
    Illegal NgoMIV site found at 6004
    Illegal AgeI site found at 1290
    Illegal AgeI site found at 1438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1117
    Illegal BsaI.rc site found at 5718
    Illegal SapI site found at 323