Difference between revisions of "Part:BBa K4129122"

Revision as of 12:55, 12 October 2022

The fungal synthetic expression system with FunsTF04 and 6xLexO-Pmin

Usage and Biology

This composite part was used to characterise the synthetic transcription factor (sTF) FunsTF04. The reporter cassette consists of the synthetic 6xLexO-Pmin (BBa_K4129120) that transcribe mCherry. For characterisation look up BBa_K4129102.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 4579
Illegal XbaI site found at 6492
Illegal PstI site found at 141
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 911
Illegal PstI site found at 141
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 145
Illegal BglII site found at 3258
Illegal BamHI site found at 2920
Illegal BamHI site found at 3917
Illegal BamHI site found at 5773
Illegal XhoI site found at 701
Illegal XhoI site found at 1083
Illegal XhoI site found at 3113
Illegal XhoI site found at 3550
Illegal XhoI site found at 4066
Illegal XhoI site found at 4443
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 4579
Illegal XbaI site found at 6492
Illegal PstI site found at 141
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 4579
Illegal XbaI site found at 6492
Illegal PstI site found at 141
Illegal NgoMIV site found at 6409
Illegal AgeI site found at 1290
Illegal AgeI site found at 1438
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1117
Illegal BsaI.rc site found at 6123
Illegal SapI site found at 323

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-This composite part was used to characterise the synthetic transcription factor (sTF) FunsTF04. The reporter cassette consists of the synthetic 6xLexO-Pmin (BBa_K4129120) that transcribe mCherry.
+This composite part was used to characterise the synthetic transcription factor (sTF) FunsTF04. The reporter cassette consists of the synthetic 6xLexO-Pmin (BBa_K4129120) that transcribe mCherry. For characterisation look up BBa_K4129102.
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