Difference between revisions of "Part:BBa K4438804"
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<partinfo>BBa_K4438804 short</partinfo> | <partinfo>BBa_K4438804 short</partinfo> | ||
| − | MN4_Target_2(BBa_K4438804) is a single-stranded DNA sequence having 84 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. The 3’ end has few bases complementary to MN4_trigger_2_w/oPol(BBa_K4438803), the middle region has ssDNA antisense T7 promoter sequence and the 5’ end has broccoli cDNA (antisense) sequence. | + | MN4_Target_2(<partinfo>BBa_K4438804</partinfo>) is a single-stranded DNA sequence having 84 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. The 3’ end has few bases complementary to MN4_trigger_2_w/oPol(<partinfo>BBa_K4438803</partinfo>), the middle region has ssDNA antisense T7 promoter sequence and the 5’ end has broccoli cDNA (antisense) sequence. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part along with MN4_trigger_2_w/oPol(BBa_K4438803) and Aptamer_MN4(BBa_K4438800) can be used to detect different concentrations of quinine [1]. | + | This part along with MN4_trigger_2_w/oPol(<partinfo>BBa_K4438803</partinfo>) and Aptamer_MN4(<partinfo>BBa_K4438800</partinfo>) can be used to detect different concentrations of quinine [1]. |
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| + | [[File:T--IISER-Tirupati_India--mn4structure2.png]] | ||
Latest revision as of 12:52, 12 October 2022
MN4_Target_2
MN4_Target_2(BBa_K4438804) is a single-stranded DNA sequence having 84 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. The 3’ end has few bases complementary to MN4_trigger_2_w/oPol(BBa_K4438803), the middle region has ssDNA antisense T7 promoter sequence and the 5’ end has broccoli cDNA (antisense) sequence.
Usage and Biology
This part along with MN4_trigger_2_w/oPol(BBa_K4438803) and Aptamer_MN4(BBa_K4438800) can be used to detect different concentrations of quinine [1].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.