Difference between revisions of "Part:BBa K4229009"

Latest revision as of 12:45, 12 October 2022

SnoopCatcher

The SnoopTag/Catcher system derives from a pilin of the bacterium Streptococcus pneumoniae. This system works similarly to the SpyTag/Catcher. They consist of a short amino acid sequence, and form a covalent bond between a lysine and an asparagine. In the SnoopTag/Catcher system, the lysine can be found in the tag and the asparagine in the catcher.

Since both SnoopTag and SnoopCatcher are made of canonical amino acids, they can be linked to proteins of interest of different organisms. In addition, they bind to each other extremely specifically, and the covalent bond is very stable under different pH and temperature conditions.

Via a short linker, the SnoopCatcher can be added at the C- or N-terminal to any gene of interest.

In our project, we fused the SnoopCatcher to TnaA (BioBrick BBa_K4229011) to target it into a microcompartment (wiffleball).

References [1] B. Zakeri et al., “Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin,” Proc. Natl. Acad. Sci. U. S. A., vol. 109, no. 12, 2012, doi: 10.1073/pnas.1115485109.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 130
Illegal AgeI site found at 216
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4229009 short</partinfo>
-snoopCatcher used in combination with a snooptag
+The SnoopTag/Catcher system derives from a pilin of the bacterium <i>Streptococcus pneumoniae</i>. This system works similarly to the SpyTag/Catcher. They consist of a short amino acid sequence, and form a covalent bond between a lysine and an asparagine. In the SnoopTag/Catcher system, the lysine can be found in the tag and the asparagine in the catcher.
+Since both SnoopTag and SnoopCatcher are made of canonical amino acids, they can be linked to proteins of interest of different organisms. In addition, they bind to each other extremely specifically, and the covalent bond is very stable under different pH and temperature conditions.
+Via a short linker, the SnoopCatcher can be added at the C- or N-terminal to any gene of interest.
+In our project, we fused the SnoopCatcher to TnaA (BioBrick BBa_K4229011) to target it into a microcompartment (wiffleball).
+<b>References</b>
+[1] B. Zakeri et al., “Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin,” Proc. Natl. Acad. Sci. U. S. A., vol. 109, no. 12, 2012, doi: 10.1073/pnas.1115485109.
 <!-- Add more about the biology of this part here