Difference between revisions of "Part:BBa K4367013:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Effort was put in to find a good length of the linker between dCas9 and nTEV. Also, effort was put into finding good binding sites for dCas9 with gRNA, in relation to binding strength, off-target binding, and distance between the two | + | Effort was put in to find a good length of the linker between dCas9 and nTEV. Also, effort was put into finding good binding sites for dCas9 with gRNA, in relation to binding strength, off-target binding, and distance between the two binding sites of dCas9 (The split halves of TEVp must reach each other). |
Latest revision as of 12:44, 12 October 2022
dCas9-cTEV
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4358
Illegal BamHI site found at 4687 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 14
Illegal BsaI.rc site found at 4694
Illegal SapI.rc site found at 4594
Design Notes
Effort was put in to find a good length of the linker between dCas9 and nTEV. Also, effort was put into finding good binding sites for dCas9 with gRNA, in relation to binding strength, off-target binding, and distance between the two binding sites of dCas9 (The split halves of TEVp must reach each other).
Source
dCas9 is a variant of Cas9 from the CRISPR/Cas9 system. cTEV is a half of split TEVp, which is Tobacco Etch Virus Protease.