Difference between revisions of "Part:BBa K4438802"

 
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<partinfo>BBa_K4438802 short</partinfo>
  
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MN4_Target_1(BBa_K4438802) is a single-stranded DNA sequence having 76 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. The 3’ end has ssDNA antisense T7 promoter sequence and the 5’ end has broccoli cDNA (antisense) sequence.
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===Usage and Biology===
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This part along with MN4_trigger_1-T7promoter-w/oPol(BBa_K4438801) and Aptamer_MN4(BBa_K4438800) can be used to detect different concentrations of quinine [1].
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4438802 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4438802 parameters</partinfo>
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===References===
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Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.

Revision as of 12:31, 12 October 2022

MN4_Target_1

MN4_Target_1(BBa_K4438802) is a single-stranded DNA sequence having 76 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. The 3’ end has ssDNA antisense T7 promoter sequence and the 5’ end has broccoli cDNA (antisense) sequence.


Usage and Biology

This part along with MN4_trigger_1-T7promoter-w/oPol(BBa_K4438801) and Aptamer_MN4(BBa_K4438800) can be used to detect different concentrations of quinine [1].




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.