Difference between revisions of "Part:BBa K4298002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | . | + | Pruduce enCas9-PolI5M with a linker between the two protein and SV40 NLS upstream. |
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===Source=== | ===Source=== | ||
| − | + | Parts on the plasmid pyEVR. | |
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Latest revision as of 12:24, 12 October 2022
enCas9-PolI5M
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 6414
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 6414
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4656
Illegal BamHI site found at 6674 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 6414
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 6414
Illegal NgoMIV site found at 1566
Illegal NgoMIV site found at 5567
Illegal AgeI site found at 5064
Illegal AgeI site found at 6654 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 205
Illegal SapI.rc site found at 1647
Design Notes
Pruduce enCas9-PolI5M with a linker between the two protein and SV40 NLS upstream.
Source
Parts on the plasmid pyEVR.