Difference between revisions of "Part:BBa K4388005:Experience"
(→Applications of BBa_K4388005) |
Tomaslopes (Talk | contribs) |
||
| (One intermediate revision by one other user not shown) | |||
| Line 8: | Line 8: | ||
<p>Through JUMP type IIS Level 1, this transcriptional unit (BBa_K4388005) was assembled in a one-pot digestion reaction with BsaI and ligation into pJUMP29-1A. Successful ligation and transformation were selected for by identifying non-fluorescent colonies on kanamycin agar plates (Fig. 1).</p> | <p>Through JUMP type IIS Level 1, this transcriptional unit (BBa_K4388005) was assembled in a one-pot digestion reaction with BsaI and ligation into pJUMP29-1A. Successful ligation and transformation were selected for by identifying non-fluorescent colonies on kanamycin agar plates (Fig. 1).</p> | ||
| − | + | [[File:"A E.coli high quality.png]] | |
| − | Figure 1. Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts | + | |
| + | <p>Figure 1. Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts BBa_K4388013, BBa_K4388001, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388005 in pJUMP29-1A via a one-pot BsaI digestion reaction. White (sfGPF-excised) colonies indicate successful ligation of the TU into plasmid, and successful transformation of plasmid conferring kanamycin resistance. Final level 1 plasmid constructs were designed using SnapGene.</p> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 12:23, 12 October 2022
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4388005
KCL iGEM 2022
Through JUMP type IIS Level 1, this transcriptional unit (BBa_K4388005) was assembled in a one-pot digestion reaction with BsaI and ligation into pJUMP29-1A. Successful ligation and transformation were selected for by identifying non-fluorescent colonies on kanamycin agar plates (Fig. 1).
Figure 1. Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts BBa_K4388013, BBa_K4388001, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388005 in pJUMP29-1A via a one-pot BsaI digestion reaction. White (sfGPF-excised) colonies indicate successful ligation of the TU into plasmid, and successful transformation of plasmid conferring kanamycin resistance. Final level 1 plasmid constructs were designed using SnapGene.
User Reviews
UNIQec0350cd7bf422d4-partinfo-00000000-QINU UNIQec0350cd7bf422d4-partinfo-00000001-QINU