Difference between revisions of "Part:BBa K4229063"

Latest revision as of 12:01, 12 October 2022

mVenus with N-terminal spyCatcher and RBS

mVenus2 (together with mTurqouise2,[BBa_K4229064]) was used as a reporter by fusing it with a SpyCatcher, enabling recruitment by the SpyTag. This biobrick contains the tagged mVenus and the RBS we used in our experimental setup. We used the fusion protein to test the functionality of our compartments: the minimal (BBa_K4229047) and full wiffleball (BBa_K4229049), and SPD5 (BBa_K4229078). All compartmentalisation proteins have an N-terminal SpyTag. For experimental data please refer to the data listed in Biobrick BBa_K4229066.

Principle of catching proteins via Spy/Snp-Catcher by T1 and their incorporation into the BMCs, illustrated as an example of incorporating mVenus in the full wiffleball

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1002
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 435
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4229063 short</partinfo>
-mVenus2 is with mTurqouise2(BBa_K4229065) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049) and SPD5 (BBa_K4229078). It has the spyCatcher fused on the N-terminal of the protein to make it recruitable inside those compartments.
+mVenus2 (together with mTurqouise2,[BBa_K4229064]) was used as a reporter by fusing it with a SpyCatcher, enabling recruitment by the SpyTag. This biobrick contains the tagged mVenus and the RBS we used in our experimental setup. We used the fusion protein to test the functionality of our compartments: the minimal (BBa_K4229047) and full wiffleball (BBa_K4229049), and SPD5 (BBa_K4229078). All compartmentalisation proteins have an N-terminal SpyTag. For experimental data please refer to the data listed in Biobrick BBa_K4229066.
 [[File:ReporterCatched.jpg|800px|thumb|left| Principle of catching proteins via Spy/Snp-Catcher by T1 and their incorporation into the BMCs, illustrated as an example of incorporating mVenus in the full wiffleball]]