Difference between revisions of "Part:BBa K4438702"
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K4438102 parameters</partinfo>
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<partinfo>BBa_K4438702 parameters</partinfo>
 
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===References===
 
===References===
 
Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082.
 
Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082.

Revision as of 11:59, 12 October 2022

P4G13_Target_1

P4G13_Target_1 (BBa_K4438702) is a single-stranded DNA sequence having 81 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 3’ end has few bases complementary to P4G13_trigger_1_w/oPol (BBa_K4438701). In the middle complete ssDNA antisense T7 promoter sequence is present. The 5’ end has broccoli cDNA sequence.

Usage and Biology

This part along with P4G13_trigger_1_w/oPol (BBa_K4438701) and P4G13_aptamer (BBa_K4438700) can be used for sensing different concentrations of progesterone [1].



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082.