Difference between revisions of "Part:BBa K4204019:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The GFP could be replaced by any protein in interest. | + | The GFP could be replaced by any CDS of protein in interest. Since our team needs to extract the protein to prove its efficiency, the GFP is being replaced with LysPBC5, generating BBa_K4204021 for characterization. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | The protein quality control system is from Yang et al's work [1]. Other components are from BBa_K653003. Assembled by BNDS_China 2022. | |
| − | Assembled by BNDS_China 2022 | + | |
===References=== | ===References=== | ||
| + | [1] Yang, J., Han, Y.H., Im, J. et al. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol 17, 421–427 (2021). https://doi.org/10.1038/s41589-021-00736-3 | ||
Latest revision as of 11:47, 12 October 2022
IPTG induced GFP improved with ProQC system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 316
Illegal NheI site found at 1116 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 978
Design Notes
The GFP could be replaced by any CDS of protein in interest. Since our team needs to extract the protein to prove its efficiency, the GFP is being replaced with LysPBC5, generating BBa_K4204021 for characterization.
Source
The protein quality control system is from Yang et al's work [1]. Other components are from BBa_K653003. Assembled by BNDS_China 2022.
References
[1] Yang, J., Han, Y.H., Im, J. et al. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol 17, 421–427 (2021). https://doi.org/10.1038/s41589-021-00736-3