Difference between revisions of "Part:BBa K4204013:Design"
Jianfei Song (Talk | contribs) (→References) |
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===Source=== | ===Source=== | ||
− | + | From Lee et al's work [1]. | |
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===References=== | ===References=== | ||
[1] Lee, K. O., Kong, M., Kim, I., Bai, J., Cha, S., Kim, B., Ryu, K.-S., Ryu, S., & Suh, J.-Y. (2019). Structural basis for cell-wall recognition by bacteriophage PBC5 endolysin. Structure, 27(9). https://doi.org/10.1016/j.str.2019.07.001 | [1] Lee, K. O., Kong, M., Kim, I., Bai, J., Cha, S., Kim, B., Ryu, K.-S., Ryu, S., & Suh, J.-Y. (2019). Structural basis for cell-wall recognition by bacteriophage PBC5 endolysin. Structure, 27(9). https://doi.org/10.1016/j.str.2019.07.001 |
Latest revision as of 11:23, 12 October 2022
N-terminal His-tagged LysPBC5
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 64
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 759
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 811
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The toehold switch would be at the 5' end of the mRNA with a corresponding trigger at the 3' end of the mRNA. This synthetic protein quality control system is believed to have no limitations regarding its use in other bacteria, and this system can be applied to improve the quality of recombinant protein production and also efficiencies of metabolic pathways.
Source
From Lee et al's work [1].
References
[1] Lee, K. O., Kong, M., Kim, I., Bai, J., Cha, S., Kim, B., Ryu, K.-S., Ryu, S., & Suh, J.-Y. (2019). Structural basis for cell-wall recognition by bacteriophage PBC5 endolysin. Structure, 27(9). https://doi.org/10.1016/j.str.2019.07.001