Difference between revisions of "Part:BBa K4119026"

 
 
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<partinfo>BBa_K4119026 short</partinfo>
 
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it's Pvgb-separate FNR binding site from -35->Bs2->Cpa fdx terminator
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PvgbF3/PvgbF7-Bs2-Cpa fdx terminator
  
  
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K4119026 SequenceAndFeatures</partinfo>
 
  
  
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<partinfo>BBa_K4119026 parameters</partinfo>
 
<partinfo>BBa_K4119026 parameters</partinfo>
 
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    <title>Document</title>
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    <p style="font-size: 180%; font-weight: bold;">Results:</p>
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    <p>After modification, both experimental groups had lower expression levels than the positive control group, and we found that the pMTL82151-PvgbF3-Bs2 experimental group did not achieve the purpose of the transformation. However，the pMTL82151-PvgbF7-Bs2 experimental group showed aerobic inhibition and microoxide expression characteristics.</p>
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    <p><img src="https://static.igem.wiki/teams/4119/wiki/jt-files/h2/img13.png" width="80%" height="80%"></p>
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    <div align="center">
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        <strong>Figure. Expression effect of PvgbF3-bs2, PvgbF7-bs2 and Pvgb at different oxygen concentrations</strong>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4119026 SequenceAndFeatures</partinfo>

Latest revision as of 11:21, 12 October 2022


Pvgb-separate FNR binding site from -35->Bs2->Cpa fdx terminator

PvgbF3/PvgbF7-Bs2-Cpa fdx terminator


Document

Results:

After modification, both experimental groups had lower expression levels than the positive control group, and we found that the pMTL82151-PvgbF3-Bs2 experimental group did not achieve the purpose of the transformation. However，the pMTL82151-PvgbF7-Bs2 experimental group showed aerobic inhibition and microoxide expression characteristics.

Figure. Expression effect of PvgbF3-bs2, PvgbF7-bs2 and Pvgb at different oxygen concentrations

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 357
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 357
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 357
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 357
  • 1000
    COMPATIBLE WITH RFC[1000]