Difference between revisions of "Part:BBa K4438114"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part can be used for the detection of miR-27a-5p, which proves to be a biomarker for many diseases. The | + | This part can be used for the detection of miR-27a-5p, which proves to be a biomarker for many diseases. The OFF(A) and ON(B) structure for the sensor was generated as given: |
[[File:T--IISER-Tirupati_India--miRNA_27a5p_2.png]] | [[File:T--IISER-Tirupati_India--miRNA_27a5p_2.png]] | ||
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Latest revision as of 11:16, 12 October 2022
FASTmiR-27a-D2
FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. FASTmiR-27a-D2, after transcription, binds to miR27a-5p, the transcription product of BBa_K4438152. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.
Usage and Biology
This part can be used for the detection of miR-27a-5p, which proves to be a biomarker for many diseases. The OFF(A) and ON(B) structure for the sensor was generated as given:
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 50
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 50
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 50
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 50
- 1000COMPATIBLE WITH RFC[1000]
Notes
The split position of the complementary sequence in the sensor is TGCTCACAAGC….AGCTAAGCCCT
References
Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130