Difference between revisions of "Part:BBa K4204014:Design"
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===References===
 
===References===
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[1]Yang J, Han YH, Im J, Seo SW. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol. 2021 Apr;17(4):421-427. doi: 10.1038/s41589-021-00736-3. Epub 2021 Feb 4. PMID: 33542534.
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[1] Yang J, Han YH, Im J, Seo SW. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol. 2021 Apr;17(4):421-427. doi: 10.1038/s41589-021-00736-3. Epub 2021 Feb 4. PMID: 33542534.
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[2]  Lee, K. O., Kong, M., Kim, I., Bai, J., Cha, S., Kim, B., Ryu, K.-S., Ryu, S., & Suh, J.-Y. (2019). Structural basis for cell-wall recognition by bacteriophage PBC5 endolysin. Structure, 27(9). https://doi.org/10.1016/j.str.2019.07.001

Latest revision as of 10:59, 12 October 2022


Quality control system + LysPBC5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 907
    Illegal PstI site found at 31
    Illegal PstI site found at 367
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 907
    Illegal NheI site found at 1017
    Illegal NheI site found at 1099
    Illegal PstI site found at 31
    Illegal PstI site found at 367
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 907
    Illegal BglII site found at 916
    Illegal BamHI site found at 1794
    Illegal XhoI site found at 2122
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 907
    Illegal PstI site found at 31
    Illegal PstI site found at 367
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 907
    Illegal PstI site found at 31
    Illegal PstI site found at 367
    Illegal NgoMIV site found at 346
    Illegal NgoMIV site found at 1846
    Illegal AgeI site found at 496
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The toehold switch would be at the 5' end of the mRNA with a corresponding trigger at the 3' end of the mRNA. This synthetic protein quality control system is believed to have no limitations regarding its use in other bacteria, and this system can be applied to improve the quality of recombinant protein production and also efficiencies of metabolic pathways.

Source

Eukaryotic translation system.

References

[1] Yang J, Han YH, Im J, Seo SW. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol. 2021 Apr;17(4):421-427. doi: 10.1038/s41589-021-00736-3. Epub 2021 Feb 4. PMID: 33542534.

[2] Lee, K. O., Kong, M., Kim, I., Bai, J., Cha, S., Kim, B., Ryu, K.-S., Ryu, S., & Suh, J.-Y. (2019). Structural basis for cell-wall recognition by bacteriophage PBC5 endolysin. Structure, 27(9). https://doi.org/10.1016/j.str.2019.07.001