Difference between revisions of "Part:BBa K4501019:Experience"
Paumarin99 (Talk | contribs) |
|||
| Line 5: | Line 5: | ||
===Applications of BBa_K4501019=== | ===Applications of BBa_K4501019=== | ||
| + | We demonstrated the downregulation of myc by transiently transfecting (by electroporation) these shRNA in a expression cassette on Ramos and HEK293T cell lines. qPCR from total RNA extraction shows as high as almost 2-fold decrease in myc expression when treatment with shRNA. | ||
| + | |||
| + | [[File:ShRNA_qPCR_complete.jpg]] | ||
| + | Figure 1. (A) Results of qPCR for Ramos and HEK293T cell line. (B) GFP expression of the cell lines transfected with the constructions. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 10:57, 12 October 2022
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4501019
We demonstrated the downregulation of myc by transiently transfecting (by electroporation) these shRNA in a expression cassette on Ramos and HEK293T cell lines. qPCR from total RNA extraction shows as high as almost 2-fold decrease in myc expression when treatment with shRNA.
Figure 1. (A) Results of qPCR for Ramos and HEK293T cell line. (B) GFP expression of the cell lines transfected with the constructions.
User Reviews
UNIQ5043720dc59e0ace-partinfo-00000000-QINU UNIQ5043720dc59e0ace-partinfo-00000001-QINU