Difference between revisions of "Part:BBa K4119010"

 
 
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K4119010 parameters</partinfo>
 
<partinfo>BBa_K4119010 parameters</partinfo>
 
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    <meta http-equiv="X-UA-Compatible" content="IE=edge">
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    <meta name="viewport" content="width=device-width, initial-scale=1.0">
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    <title>Document</title>
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<body>
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    <p style="font-size: 180%; font-weight: bold;">Results:</p>
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    <p><img src="https://static.igem.wiki/teams/4119/wiki/jt-files/h2/img9.png" width="80%" height="80%"></p>
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    <div align="center">
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        <strong>Fig-2 Plasmid-self-target bacteria colonies PCR results</strong>
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    </div>
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    <p>The results show that the target plasmid colonies PCR results appear band to eliminate the plasmid bacteria no
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        band, at the same time, eliminate the target plasmid bacteria cannot survive in the medium containing
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        resistance, prove that the target bacteria has completed plasmid elimination.</p>
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    <p>We hope to induce the elimination of plasmids by tetracycline after lactose-induced knockout of the gene of
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        interest, and at present, we have only achieved lactose-induced knockout of the gene of interest with success.
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        In addition, we designed plasmids solely to eliminate plasmids to verify whether the method of eliminating
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        plasmids using tetr is feasible in Clostridium butyrate. We introduced a plasmid carrying the tetR gene in
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        Clostridium butyrate and successfully eliminated it by tetracycline induction, demonstrating that this method is
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        feasible. The work of knocking out the gene of interest and then eliminating the plasmid for time reasons is not
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        completed. </p>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4119010 SequenceAndFeatures</partinfo>

Latest revision as of 10:55, 12 October 2022


Plac->ΔperR->self-target->Cpa fdx terminator

it's Plac->ΔperR->self-target->Cpa fdx terminator


Document

Results:

Fig-2 Plasmid-self-target bacteria colonies PCR results

The results show that the target plasmid colonies PCR results appear band to eliminate the plasmid bacteria no band, at the same time, eliminate the target plasmid bacteria cannot survive in the medium containing resistance, prove that the target bacteria has completed plasmid elimination.

We hope to induce the elimination of plasmids by tetracycline after lactose-induced knockout of the gene of interest, and at present, we have only achieved lactose-induced knockout of the gene of interest with success. In addition, we designed plasmids solely to eliminate plasmids to verify whether the method of eliminating plasmids using tetr is feasible in Clostridium butyrate. We introduced a plasmid carrying the tetR gene in Clostridium butyrate and successfully eliminated it by tetracycline induction, demonstrating that this method is feasible. The work of knocking out the gene of interest and then eliminating the plasmid for time reasons is not completed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 368
    Illegal PstI site found at 1597
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 368
    Illegal PstI site found at 1597
    Illegal NotI site found at 3487
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 368
    Illegal BglII site found at 428
    Illegal XhoI site found at 3359
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 368
    Illegal PstI site found at 1597
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 368
    Illegal PstI site found at 1597
    Illegal NgoMIV site found at 3791
  • 1000
    COMPATIBLE WITH RFC[1000]