Difference between revisions of "Part:BBa K4501000:Experience"
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===Applications of BBa_K4501000=== | ===Applications of BBa_K4501000=== | ||
| + | We demonstrated the downregulation of myc by transiently transfecting (by electroporation) these shRNA in a expression cassette on Ramos and HEK293T cell lines. qPCR from total RNA extraction shows as high as almost 2-fold decrease in myc expression when treatment with shRNA. | ||
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| + | [[File:ShRNA_qPCR.jpg]] | ||
| + | Figure 1. Results of qPCR for Ramos and HEK293T cell line. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 10:54, 12 October 2022
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Applications of BBa_K4501000
We demonstrated the downregulation of myc by transiently transfecting (by electroporation) these shRNA in a expression cassette on Ramos and HEK293T cell lines. qPCR from total RNA extraction shows as high as almost 2-fold decrease in myc expression when treatment with shRNA.
Figure 1. Results of qPCR for Ramos and HEK293T cell line.
User Reviews
UNIQb8ab15b0369cbca2-partinfo-00000000-QINU UNIQb8ab15b0369cbca2-partinfo-00000001-QINU