Difference between revisions of "Part:BBa K4501002:Experience"
Paumarin99 (Talk | contribs) |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 5: | Line 5: | ||
===Applications of BBa_K4501002=== | ===Applications of BBa_K4501002=== | ||
| + | We demonstrated the downregulation of myc by transiently transfecting (by electroporation) these shRNA in a expression cassette on Ramos and HEK293T cell lines. qPCR from total RNA extraction shows as high as almost 2-fold decrease in myc expression when treatment with shRNA. | ||
| + | |||
| + | [[File:ShRNA_qPCR.jpg]] | ||
| + | Figure 1. Results of qPCR for Ramos and HEK293T cell line. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 10:53, 12 October 2022
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4501002
We demonstrated the downregulation of myc by transiently transfecting (by electroporation) these shRNA in a expression cassette on Ramos and HEK293T cell lines. qPCR from total RNA extraction shows as high as almost 2-fold decrease in myc expression when treatment with shRNA.
Figure 1. Results of qPCR for Ramos and HEK293T cell line.
User Reviews
UNIQ385bfc7749db85a9-partinfo-00000000-QINU UNIQ385bfc7749db85a9-partinfo-00000001-QINU