Difference between revisions of "Part:BBa K4204003"
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| + | ===Transcriptional unit of sgRNA targeting HBLA gene of B.cereus=== | ||
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==Description== | ==Description== | ||
This sequence contains 3 protection bases at the 5’ end of the sequence, which is to protect the sequence from damage. And immediately following the T7 promoter， connecting with the sgRNA scaffold, which helps the folding of sgRNA. And at the 3’ end, the target-HBLA region pairs with the targeted B.cereus HBLA gene sequence and enables cleavage. | This sequence contains 3 protection bases at the 5’ end of the sequence, which is to protect the sequence from damage. And immediately following the T7 promoter， connecting with the sgRNA scaffold, which helps the folding of sgRNA. And at the 3’ end, the target-HBLA region pairs with the targeted B.cereus HBLA gene sequence and enables cleavage. | ||
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| + | <!-- --> | ||
| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K4204003 SequenceAndFeatures</partinfo> | ||
Latest revision as of 10:51, 12 October 2022
Transcriptional unit of sgRNA targeting HBLA gene of B.cereus
Description
This sequence contains 3 protection bases at the 5’ end of the sequence, which is to protect the sequence from damage. And immediately following the T7 promoter， connecting with the sgRNA scaffold, which helps the folding of sgRNA. And at the 3’ end, the target-HBLA region pairs with the targeted B.cereus HBLA gene sequence and enables cleavage.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 26
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 26
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 26
- 1000COMPATIBLE WITH RFC[1000]