Difference between revisions of "Part:BBa K4201021"

 
 
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<partinfo>BBa_K4201021 short</partinfo>
 
<partinfo>BBa_K4201021 short</partinfo>
  
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This construct contains four main segments: CrtE, cytoTDS2-MBP, T5&#945;OH, and RUBY. Each segment encodes for a particular gene(s) of interest, and is enclosed by an upstream Gmubi promoter and downstream AtHSP terminator.
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This construct contains four main segments: CrtE, cytoTDS2-MBP, T5&#945;OH, and RUBY. Each segment encodes for a particular gene(s) of interest, and is enclosed by an upstream Gmubi promoter and downstream AtHSP terminator. CrtE encodes for a GGPP synthase that converts DMAPP into GGPP1, a precursor in the paclitaxel pathway<sup>1</sup>. Taxadiene synthase II, an enzyme encoded by cytoTDS2, then converts the GGPP into taxadiene<sup>2</sup>. This taxadiene is used by T5αOH to synthesize Taxadiene-5α-ol, the next molecule in the paclitaxel pathway. <sup>3</sup>
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Maltose binding protein (MBP) is known as a “fusion partner,” which is used for producing recombinant proteins in bacterial cells. It also has a high rate of translation, and ensures the proper folding and solubility of the target protein<sup>4</sup>.  
  
 
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Latest revision as of 10:46, 12 October 2022


CrtE_cytoTDS2-MBP_T5αOH_RUBY

This construct contains four main segments: CrtE, cytoTDS2-MBP, T5αOH, and RUBY. Each segment encodes for a particular gene(s) of interest, and is enclosed by an upstream Gmubi promoter and downstream AtHSP terminator. CrtE encodes for a GGPP synthase that converts DMAPP into GGPP1, a precursor in the paclitaxel pathway1. Taxadiene synthase II, an enzyme encoded by cytoTDS2, then converts the GGPP into taxadiene2. This taxadiene is used by T5αOH to synthesize Taxadiene-5α-ol, the next molecule in the paclitaxel pathway. 3

Maltose binding protein (MBP) is known as a “fusion partner,” which is used for producing recombinant proteins in bacterial cells. It also has a high rate of translation, and ensures the proper folding and solubility of the target protein4.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 8103
    Illegal XbaI site found at 4087
    Illegal PstI site found at 2087
    Illegal PstI site found at 3774
    Illegal PstI site found at 5335
    Illegal PstI site found at 6893
    Illegal PstI site found at 9650
    Illegal PstI site found at 14858
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 8103
    Illegal PstI site found at 2087
    Illegal PstI site found at 3774
    Illegal PstI site found at 5335
    Illegal PstI site found at 6893
    Illegal PstI site found at 9650
    Illegal PstI site found at 14858
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 8103
    Illegal BglII site found at 790
    Illegal BglII site found at 1153
    Illegal BglII site found at 2021
    Illegal BglII site found at 2956
    Illegal BglII site found at 6827
    Illegal BglII site found at 7762
    Illegal BglII site found at 8719
    Illegal BamHI site found at 13070
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 8103
    Illegal XbaI site found at 4087
    Illegal PstI site found at 2087
    Illegal PstI site found at 3774
    Illegal PstI site found at 5335
    Illegal PstI site found at 6893
    Illegal PstI site found at 9650
    Illegal PstI site found at 14858
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 8103
    Illegal XbaI site found at 4087
    Illegal PstI site found at 2087
    Illegal PstI site found at 3774
    Illegal PstI site found at 5335
    Illegal PstI site found at 6893
    Illegal PstI site found at 9650
    Illegal PstI site found at 14858
    Illegal NgoMIV site found at 1697
    Illegal NgoMIV site found at 8252
    Illegal NgoMIV site found at 11006
    Illegal NgoMIV site found at 11585
    Illegal NgoMIV site found at 13298
    Illegal AgeI site found at 3923
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 948
    Illegal BsaI site found at 1887
    Illegal BsaI site found at 2167
    Illegal BsaI site found at 3114
    Illegal BsaI site found at 6693
    Illegal BsaI site found at 6973
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 1873
    Illegal BsaI.rc site found at 2153
    Illegal BsaI.rc site found at 3100
    Illegal BsaI.rc site found at 6679
    Illegal BsaI.rc site found at 6959
    Illegal BsaI.rc site found at 7906