Difference between revisions of "Part:BBa K4501002"
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Encodes for an shRNA against the 1741-1761 positions of the mRNA principal transcript of human MYC (HGNC:7553). It is composed of a sense and an anti-sense sequence separated by a loop domain. To optimize and stabilize the transcript, the C/D-box sequence is allocated at the position of the loop, substituting the loop. | Encodes for an shRNA against the 1741-1761 positions of the mRNA principal transcript of human MYC (HGNC:7553). It is composed of a sense and an anti-sense sequence separated by a loop domain. To optimize and stabilize the transcript, the C/D-box sequence is allocated at the position of the loop, substituting the loop. | ||
| − | [[BBa K4501002.jpeg]] | + | |
| + | [[File:BBa K4501002.jpeg]] | ||
| + | Figure 1. Structure and stability of the shRNA | ||
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Latest revision as of 10:43, 12 October 2022
anti-myc shRNA (1741-1761) in-loop C/D-box
Encodes for an shRNA against the 1741-1761 positions of the mRNA principal transcript of human MYC (HGNC:7553). It is composed of a sense and an anti-sense sequence separated by a loop domain. To optimize and stabilize the transcript, the C/D-box sequence is allocated at the position of the loop, substituting the loop.
Figure 1. Structure and stability of the shRNA
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]