Difference between revisions of "Part:BBa K4181002"
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This plasmid, named PHT01, is an expression vector for COTE-binding protein and DSUP protein that we need to express. It can be introduced into Escherichia coli and then introduced into Bacillus subtilis for expression | This plasmid, named PHT01, is an expression vector for COTE-binding protein and DSUP protein that we need to express. It can be introduced into Escherichia coli and then introduced into Bacillus subtilis for expression | ||
| − | + | ===Plasmid profiles=== | |
| − | === | + | [[File:2022-SJTU-Biox-Shanghai-12.png|500px|center]]<br> |
| + | <p align="center">'''Figure 1.'''PDG1730<br></p> | ||
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Latest revision as of 10:40, 12 October 2022
A shuttle plasmid that functions in Bacillus subtilis and Escherichia coli
This plasmid, named PHT01, is an expression vector for COTE-binding protein and DSUP protein that we need to express. It can be introduced into Escherichia coli and then introduced into Bacillus subtilis for expression
Plasmid profiles
Figure 1.PDG1730
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1256
Illegal EcoRI site found at 3406
Illegal XbaI site found at 2810 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1256
Illegal EcoRI site found at 3406
Illegal NheI site found at 1092 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1256
Illegal EcoRI site found at 3406
Illegal BglII site found at 2877
Illegal BglII site found at 5958
Illegal BamHI site found at 2804
Illegal XhoI site found at 5962 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1256
Illegal EcoRI site found at 3406
Illegal XbaI site found at 2810 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1256
Illegal EcoRI site found at 3406
Illegal XbaI site found at 2810 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 6853
Illegal SapI.rc site found at 4030
Illegal SapI.rc site found at 7935