Difference between revisions of "Part:BBa K4201019"

Latest revision as of 10:27, 12 October 2022

crtE-cytoTDS-MBP_T5αOH_RUBY

This composite part is made using a Kan BsaI backbone. The B/W selection was excised during Golden Gate synthesis and replaced with a sequence containing CrtE-cytoTDS-MBP, T5αOH, and RUBY genes. Each inserted gene is flanked by a Gmubi promoter and AtHSP terminator. This construct was made to be transfected into agrobacterium and then into Glycine max. The Crte-cytoTDS-MBP is a fusion protein that codes for the first two enzymes in the paclitaxel biosynthesis pathway, a GGPP synthase¹ and taxadiene synthase². The T5αOH encodes for the third step in the biosynthesis of paclitaxel³. The RUBY reporter was added to visualize the construct when integrated into the G. max genome⁴

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 6826
Illegal PstI site found at 994
Illegal PstI site found at 4792
Illegal PstI site found at 5632
Illegal PstI site found at 8365
Illegal PstI site found at 13549
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 6826
Illegal PstI site found at 994
Illegal PstI site found at 4792
Illegal PstI site found at 5632
Illegal PstI site found at 8365
Illegal PstI site found at 13549
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 6826
Illegal BglII site found at 790
Illegal BglII site found at 1610
Illegal BglII site found at 3148
Illegal BglII site found at 4195
Illegal BglII site found at 5566
Illegal BglII site found at 6493
Illegal BglII site found at 7442
Illegal BamHI site found at 5246
Illegal BamHI site found at 11769
Illegal XhoI site found at 2571
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 6826
Illegal PstI site found at 994
Illegal PstI site found at 4792
Illegal PstI site found at 5632
Illegal PstI site found at 8365
Illegal PstI site found at 13549
25
INCOMPATIBLE WITH RFC[25]
Unknown
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI site found at 940
Illegal BsaI site found at 5432
Illegal BsaI site found at 5704
Illegal BsaI site found at 6643
Illegal BsaI site found at 8165
Illegal BsaI site found at 8437
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 5426
Illegal BsaI.rc site found at 5698
Illegal BsaI.rc site found at 6637
Illegal BsaI.rc site found at 8159
Illegal BsaI.rc site found at 8431
Illegal BsaI.rc site found at 9370
Illegal SapI.rc site found at 3369
Illegal SapI.rc site found at 3575

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4201019 short</partinfo>
-This composite part is made using a Kan BsaI backbone. The B/W selection was excised during Golden Gate synthesis and replaced with a sequence containing CrtE-cytoTDS-MBP, T5aOH, and RUBY genes. Each inserted gene is flanked by a Gmubi promoter and AtHSP terminator. This construct was made to be transfected into agrobacterium and then into Glycine max.
+This composite part is made using a Kan BsaI backbone. The B/W selection was excised during Golden Gate synthesis and replaced with a sequence containing CrtE-cytoTDS-MBP, T5αOH, and RUBY genes. Each inserted gene is flanked by a Gmubi promoter and AtHSP terminator. This construct was made to be transfected into agrobacterium and then into <i>Glycine max</i>.
-The Crte-cytoTDS-MBP is a fusion protein that code for the first two enzymes in the paclitaxel biosynthesis pathway. The T5aOH encodes for the third step in the biosynthesis of paclitaxel. The RUBY reporter was added to visualize the construct when integrated into the G. max genome.
+The Crte-cytoTDS-MBP is a fusion protein that codes for the first two enzymes in the paclitaxel biosynthesis pathway, a GGPP synthase<sup>1</sup> and taxadiene synthase<sup>2</sup>. The T5αOH encodes for the third step in the biosynthesis of paclitaxel<sup>3</sup>. The RUBY reporter was added to visualize the construct when integrated into the <i>G. max</i> genome<sup>4</sup>
 <!-- Add more about the biology of this part here