Difference between revisions of "Part:BBa K4488003"
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Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. GenBank ID: AIF28006 | Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. GenBank ID: AIF28006 | ||
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| + | <b>Usage</b> Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488029 | ||
[[File:modelling-sf-6.gif|centre|thumb|700px| Modelling of Nanobody 6 (AIF28006) to sfGFP made using python package Lightdock]] | [[File:modelling-sf-6.gif|centre|thumb|700px| Modelling of Nanobody 6 (AIF28006) to sfGFP made using python package Lightdock]] | ||
Latest revision as of 10:12, 12 October 2022
Anti-GFP Nanobody/sdAb
Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. GenBank ID: AIF28006
Usage Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488029
References: Twair, A., Al-Okla, S., Zarkawi, M., & Abbady, A. Q. (2014). Characterization of camel nanobodies specific for superfolder GFP fusion proteins. Molecular biology reports, 41(10), 6887–6898. https://doi.org/10.1007/s11033-014-3575-x
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]