Difference between revisions of "Part:BBa K4501006"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part can be used as the 3' module to be assembled into the pJUMP backbones and transform them into a lentiviral transfer plasmid, together with the 5' module (BBa_K4501005) | ||
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Latest revision as of 10:12, 12 October 2022
MSCV 3' module
This module contains the necessary elements for the 3' region upstream of a 3rd generation lentiviral transfer plasmid. Encodes for the 3' LTR as well as for the SV40 polyadenylation signal. Additionally, it contains the SV40 Origen of replication (ORI), which increases the transfection efficacy in the packaging cell line expressing the SV40 peptide such as the widely used HEK293T cell line. This part is to be incorporated on the BbsI secondary assembly site of pJUMP backbones via BbsI adapters (not included in the sequence).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 33
Illegal BglII site found at 74 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]