Difference between revisions of "Part:BBa K4501005"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | This part can be used as the 5' module to be assembled into the pJUMP backbones and transform them into a lentiviral transfer plasmid, together with the 3' module (BBa_K4501006) | ||
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Latest revision as of 10:11, 12 October 2022
MSCV 5' module
This 5' module codifies for the main 5' upstream components needed in a lentiviral transfer plasmid. Spceifically, it encodes for the T3 and RSV promoters, 5' LTR, psi sequence, RRE and gp41 peptide. The 5' LTR acts as an RNA polymerase II promoter, initiating the transcription of the transgene. The psi sequence is a RNA target site for packaging by the nucleocapsid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 268
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 780