Difference between revisions of "Part:BBa K4201017"
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This construct is split up into three main gene segments. At the center of each segment lies a gene of interest, in this case CrtE, cytoTDS2, and RUBY, respectively. Each segment is flanked upstream by the promoter Gmubi and downstream by the terminator AtHSP. CrtE encodes for an enzyme that synthesizes GGPP<sup>1</sup>. The GGPP is then used to make taxadiene by taxadiene synthase, encoded by cytoTDS2<sup>2</sup>. | This construct is split up into three main gene segments. At the center of each segment lies a gene of interest, in this case CrtE, cytoTDS2, and RUBY, respectively. Each segment is flanked upstream by the promoter Gmubi and downstream by the terminator AtHSP. CrtE encodes for an enzyme that synthesizes GGPP<sup>1</sup>. The GGPP is then used to make taxadiene by taxadiene synthase, encoded by cytoTDS2<sup>2</sup>. | ||
+ | RUBY is a reporter gene that helps visualize transformation by turning transformed cells purple-red in color<sup>3</sup>. | ||
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Latest revision as of 10:08, 12 October 2022
CrtE_cytoTDS2_RUBY
This construct is split up into three main gene segments. At the center of each segment lies a gene of interest, in this case CrtE, cytoTDS2, and RUBY, respectively. Each segment is flanked upstream by the promoter Gmubi and downstream by the terminator AtHSP. CrtE encodes for an enzyme that synthesizes GGPP1. The GGPP is then used to make taxadiene by taxadiene synthase, encoded by cytoTDS22.
RUBY is a reporter gene that helps visualize transformation by turning transformed cells purple-red in color3.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2087
Illegal PstI site found at 5638
Illegal PstI site found at 10846 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2087
Illegal PstI site found at 5638
Illegal PstI site found at 10846 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 790
Illegal BglII site found at 1153
Illegal BglII site found at 2021
Illegal BglII site found at 2956
Illegal BglII site found at 4011
Illegal BglII site found at 5324
Illegal BglII site found at 5572
Illegal BamHI site found at 9058
Illegal XhoI site found at 3601
Illegal XhoI site found at 3868 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2087
Illegal PstI site found at 5638
Illegal PstI site found at 10846 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2087
Illegal PstI site found at 5638
Illegal PstI site found at 10846
Illegal NgoMIV site found at 1697
Illegal NgoMIV site found at 6994
Illegal NgoMIV site found at 7573
Illegal NgoMIV site found at 9286 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 948
Illegal BsaI site found at 1887
Illegal BsaI site found at 2167
Illegal BsaI site found at 3113
Illegal BsaI site found at 5438
Illegal BsaI site found at 5718
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 1873
Illegal BsaI.rc site found at 2153
Illegal BsaI.rc site found at 3100
Illegal BsaI.rc site found at 5424
Illegal BsaI.rc site found at 5704
Illegal BsaI.rc site found at 6651