Difference between revisions of "Part:BBa K4201017"

 
 
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<partinfo>BBa_K4201017 short</partinfo>
 
<partinfo>BBa_K4201017 short</partinfo>
  
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This construct is split up into three main gene segments. At the center of each segment lies a gene of interest, in this case CrtE, cytoTDS2, and RUBY, respectively. Each segment is flanked upstream by the promoter Gmubi and downstream by the terminator AtHSP. CrtE encodes for an enzyme that synthesizes GGPP<sup>1</sup>. The GGPP is then used to make taxadiene by taxadiene synthase, encoded by cytoTDS2<sup>2</sup>.
  
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RUBY is a reporter gene that helps visualize transformation by turning transformed cells purple-red in color<sup>3</sup>.
  
 
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Latest revision as of 10:08, 12 October 2022


CrtE_cytoTDS2_RUBY

This construct is split up into three main gene segments. At the center of each segment lies a gene of interest, in this case CrtE, cytoTDS2, and RUBY, respectively. Each segment is flanked upstream by the promoter Gmubi and downstream by the terminator AtHSP. CrtE encodes for an enzyme that synthesizes GGPP1. The GGPP is then used to make taxadiene by taxadiene synthase, encoded by cytoTDS22.

RUBY is a reporter gene that helps visualize transformation by turning transformed cells purple-red in color3.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 10846
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 10846
    Illegal NgoMIV site found at 1697
    Illegal NgoMIV site found at 6994
    Illegal NgoMIV site found at 7573
    Illegal NgoMIV site found at 9286
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 948
    Illegal BsaI site found at 1887
    Illegal BsaI site found at 2167
    Illegal BsaI site found at 3113
    Illegal BsaI site found at 5438
    Illegal BsaI site found at 5718
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 1873
    Illegal BsaI.rc site found at 2153
    Illegal BsaI.rc site found at 3100
    Illegal BsaI.rc site found at 5424
    Illegal BsaI.rc site found at 5704
    Illegal BsaI.rc site found at 6651