Difference between revisions of "Part:BBa K4488001"
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<partinfo>BBa_K4488001 short</partinfo> | <partinfo>BBa_K4488001 short</partinfo> | ||
| − | Nanobody binding | + | Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. GenBank ID: AIF28002 with minor edits. |
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| + | <b>Usage</b> | ||
| + | Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488031 | ||
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| + | [[File:modelling-sf-2.gif|centre|thumb|700px| Modelling of binding of Nanobody 2 (AIF28002) to sfGFP made using python package LightDock]] | ||
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| + | References: | ||
| + | Twair, A., Al-Okla, S., Zarkawi, M., & Abbady, A. Q. (2014). Characterization of camel nanobodies specific for superfolder GFP fusion proteins. Molecular biology reports, 41(10), 6887–6898. https://doi.org/10.1007/s11033-014-3575-x | ||
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Latest revision as of 10:01, 12 October 2022
Anti-GFP Nanobody/sdAb
Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. GenBank ID: AIF28002 with minor edits.
Usage Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488031
References: Twair, A., Al-Okla, S., Zarkawi, M., & Abbady, A. Q. (2014). Characterization of camel nanobodies specific for superfolder GFP fusion proteins. Molecular biology reports, 41(10), 6887–6898. https://doi.org/10.1007/s11033-014-3575-x
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]