Difference between revisions of "Part:BBa K4201020:Design"
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===Source=== | ===Source=== | ||
− | CrtE is a GGPP synthase from <i> Pantoea ananatis LMG 20103 </i> | + | CrtE is a GGPP synthase from <i> Pantoea ananatis LMG 20103</i><sup>1</sup>. |
− | + | Gmubi promoters originate from <i>Glycine max</i><sup>5</sup>. | |
− | + | AtHSP terminators originate from the <i>Arabidopsis thaliana</i> genome<sup>6</sup>. | |
− | + | cytoTDS2 is a taxadiene synthase native to <i>Taxus chinensis var. mairei</i> optimized for use in <i>Glycine max</i><sup>2</sup>. | |
− | + | T5αOH is a hydroxylase from <i>Taxus baccata</i> that converts taxadiene into taxadiene-5α-ol, a step in the paclitaxel pathway<sup>3</sup>. | |
− | + | RUBY is a reporter gene from the order Caryophyllales<sup>4</sup>. | |
<i>de novo</i> Synthesis was completed by iGem sponsors IDT and Twist Biosciences. | <i>de novo</i> Synthesis was completed by iGem sponsors IDT and Twist Biosciences. |
Revision as of 09:49, 12 October 2022
CrtE_cytoTDS2__T5αOH_RUBY
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Design Notes
Part information and design consideration can be found on respective basic parts pages.
In contrast to other composite parts designed by our group, each individual gene in this assembly has its own promoter and terminator, which allows us to study and compare which combinations of adjoining or separated genes results in the best expression of our target genes.
Gmubi is constitutive promoter native to Glyine max5, while AtHSP is the terminator of a heat shock protein that has shown to promote expression in plants6.
Source
CrtE is a GGPP synthase from Pantoea ananatis LMG 201031.
Gmubi promoters originate from Glycine max5.
AtHSP terminators originate from the Arabidopsis thaliana genome6.
cytoTDS2 is a taxadiene synthase native to Taxus chinensis var. mairei optimized for use in Glycine max2.
T5αOH is a hydroxylase from Taxus baccata that converts taxadiene into taxadiene-5α-ol, a step in the paclitaxel pathway3.
RUBY is a reporter gene from the order Caryophyllales4.
de novo Synthesis was completed by iGem sponsors IDT and Twist Biosciences.