Difference between revisions of "Part:BBa K4438126"
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<partinfo>BBa_K4438126 short</partinfo> | <partinfo>BBa_K4438126 short</partinfo> | ||
| − | FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is an expression cassette for BBa_K4438106, with BBa_J64997 upstream and BBa_K731721 downstream, along with the spacer sequences BBa_K3831027(at 5’ end) and BBa_K4438136(at 3’end). FASTmiR-146a-D2_s, after transcription, binds to miR-146a, the transcription product of BBa_K4438501. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence. | + | FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is an expression cassette for <partinfo>BBa_K4438106</partinfo>, with <partinfo>BBa_J64997</partinfo> upstream and <partinfo>BBa_K731721</partinfo> downstream, along with the spacer sequences <partinfo>BBa_K3831027</partinfo>(at 5’ end) and <partinfo>BBa_K4438136</partinfo>(at 3’end). FASTmiR-146a-D2_s, after transcription, binds to miR-146a, the transcription product of <partinfo>BBa_K4438501</partinfo>. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence. |
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This part can be used for the detection of miR-146a, which proves to be a biomarker for many diseases. A promoter sequence added in the beginning enhances its usage in both in-vivo and in-vitro conditions. The ON and OFF structure for the sensor was generated as given: | This part can be used for the detection of miR-146a, which proves to be a biomarker for many diseases. A promoter sequence added in the beginning enhances its usage in both in-vivo and in-vitro conditions. The ON and OFF structure for the sensor was generated as given: | ||
| − | + | [[File:T--IISER-Tirupati_India--miRNA_146_2.png]] | |
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Revision as of 09:35, 12 October 2022
FASTmiR-146a-D2_s
FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is an expression cassette for BBa_K4438106, with BBa_J64997 upstream and BBa_K731721 downstream, along with the spacer sequences BBa_K3831027(at 5’ end) and BBa_K4438136(at 3’end). FASTmiR-146a-D2_s, after transcription, binds to miR-146a, the transcription product of No part name specified with partinfo tag.. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.
Usage and Biology
This part can be used for the detection of miR-146a, which proves to be a biomarker for many diseases. A promoter sequence added in the beginning enhances its usage in both in-vivo and in-vitro conditions. The ON and OFF structure for the sensor was generated as given:
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 101
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 177
Illegal SpeI site found at 101 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 101
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 101
- 1000COMPATIBLE WITH RFC[1000]
Notes
The split position of the complementary sequence in the sensor is CTGAAGAACTGA….ATTTCAGAGG
References
Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130