Difference between revisions of "Part:BBa K4201020"
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<partinfo>BBa_K4201020 short</partinfo> | <partinfo>BBa_K4201020 short</partinfo> | ||
− | This construct is made of four separate segments: CrtE, cytoTDS2, T5αOH, and RUBY. Each segment is enclosed by an upstream Gmubi promoter and downstream AtHSP terminator. | + | This construct is made of four separate segments: CrtE, cytoTDS2, T5αOH, and RUBY. Each segment is enclosed by an upstream Gmubi promoter and downstream AtHSP terminator. CrtE encodes for a GGPP synthase that converts DMAPP into GGPP<sup>1</sup>, a precursor in the paclitaxel pathway. |
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+ | The construct is made to induce the biosynthesis of taxadiene, and consequently Taxadiene-5α-ol, in <i>Glycine max</i>. | ||
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Revision as of 09:31, 12 October 2022
CrtE_cytoTDS2__T5αOH_RUBY
This construct is made of four separate segments: CrtE, cytoTDS2, T5αOH, and RUBY. Each segment is enclosed by an upstream Gmubi promoter and downstream AtHSP terminator. CrtE encodes for a GGPP synthase that converts DMAPP into GGPP1, a precursor in the paclitaxel pathway.
The construct is made to induce the biosynthesis of taxadiene, and consequently Taxadiene-5α-ol, in Glycine max.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6848
Illegal PstI site found at 2087
Illegal PstI site found at 5638
Illegal PstI site found at 8395
Illegal PstI site found at 13603 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6848
Illegal PstI site found at 2087
Illegal PstI site found at 5638
Illegal PstI site found at 8395
Illegal PstI site found at 13603 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6848
Illegal BglII site found at 790
Illegal BglII site found at 1153
Illegal BglII site found at 2021
Illegal BglII site found at 2956
Illegal BglII site found at 4011
Illegal BglII site found at 5324
Illegal BglII site found at 5572
Illegal BamHI site found at 11815
Illegal XhoI site found at 3601
Illegal XhoI site found at 3868 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6848
Illegal PstI site found at 2087
Illegal PstI site found at 5638
Illegal PstI site found at 8395
Illegal PstI site found at 13603 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6848
Illegal PstI site found at 2087
Illegal PstI site found at 5638
Illegal PstI site found at 8395
Illegal PstI site found at 13603
Illegal NgoMIV site found at 1697
Illegal NgoMIV site found at 6997
Illegal NgoMIV site found at 9751
Illegal NgoMIV site found at 10330
Illegal NgoMIV site found at 12043 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 948
Illegal BsaI site found at 1887
Illegal BsaI site found at 2167
Illegal BsaI site found at 3113
Illegal BsaI site found at 5438
Illegal BsaI site found at 5718
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 1873
Illegal BsaI.rc site found at 2153
Illegal BsaI.rc site found at 3100
Illegal BsaI.rc site found at 5424
Illegal BsaI.rc site found at 5704
Illegal BsaI.rc site found at 6651