Difference between revisions of "Part:BBa K4477010:Design"

 
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===Design Notes===
 
===Design Notes===
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We used a pre-existing RBS, BBa_J61100, as a starting point for optimization with Salis. We aimed for 75% relative expression so as to get a high level of protein expression of BBa_K4477001 without overburdening the E. coli expressing the gene. We optimized the RBS sequence to exclude restriction sites incompatible with various assembly methods.
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We used a pre-existing RBS, BBa_J61100, as a starting point for optimization with Salis. We aimed for 75% relative expression so as to achieve a high level of protein expression of BBa_K4477005 without overburdening the E. coli expressing the gene. We optimized the RBS sequence to exclude restriction sites incompatible with various assembly methods.
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===Source===
 
===Source===

Latest revision as of 08:51, 12 October 2022


RBS for camelid anti-mouse VHH expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used a pre-existing RBS, BBa_J61100, as a starting point for optimization with Salis. We aimed for 75% relative expression so as to achieve a high level of protein expression of BBa_K4477005 without overburdening the E. coli expressing the gene. We optimized the RBS sequence to exclude restriction sites incompatible with various assembly methods.

Source

BBa_J61100 https://salislab.net/software/

References