Difference between revisions of "Part:BBa K4201016:Design"
(→Design Notes) |
(→Design Notes) |
||
Line 9: | Line 9: | ||
Part information and design consideration can be found on respective basic parts pages. | Part information and design consideration can be found on respective basic parts pages. | ||
− | This construct differs from similar composite parts like | + | This construct differs from similar composite parts like BBa_K4201018 by having different promoter (Gmubi) and terminator (AtHSP) amounts and locations. In these constructs, dashes(-) represent locations where genes were synthesized <i>de novo</i>. An underscore (_) symbolizes a location where we adhered genes together via Golden Gate assembly. Utilizing Golden Gate assembly allowed us to insert promoter and terminator sequences between genes of interest, and consequently enables the study of transcription efficacy based on promoter and terminator location. |
===Source=== | ===Source=== |
Revision as of 08:38, 12 October 2022
Crte-cytoTDS-MBP_RUBY
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1002
Illegal PstI site found at 4800
Illegal PstI site found at 5648
Illegal PstI site found at 10856
Illegal NgoMIV site found at 7004
Illegal NgoMIV site found at 7583
Illegal NgoMIV site found at 9296
Illegal AgeI site found at 2321 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 948
Illegal BsaI site found at 5448
Illegal BsaI site found at 5728
Illegal BsaI site found at 6675
Illegal BsaI site found at 10656
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 5434
Illegal BsaI.rc site found at 5714
Illegal BsaI.rc site found at 6661
Illegal BsaI.rc site found at 10642
Illegal BsaI.rc site found at 10922
Illegal SapI.rc site found at 3377
Illegal SapI.rc site found at 3583
Design Notes
Part information and design consideration can be found on respective basic parts pages.
This construct differs from similar composite parts like BBa_K4201018 by having different promoter (Gmubi) and terminator (AtHSP) amounts and locations. In these constructs, dashes(-) represent locations where genes were synthesized de novo. An underscore (_) symbolizes a location where we adhered genes together via Golden Gate assembly. Utilizing Golden Gate assembly allowed us to insert promoter and terminator sequences between genes of interest, and consequently enables the study of transcription efficacy based on promoter and terminator location.
Source
Crte-cytoTDS-MBP is a de novo synthesized construct made for this project. Learn more about our construct and its genes by searching for BBa_K4201004.
RUBY is a reporter gene from the order Caryophyllales.
Gmubi promoters originate from Glycine max.
AtHSP terminators originate from the Arabidopsis thaliana genome.
Synthesis was completed by iGem sponsors IDT and Twist Biosciences.