Difference between revisions of "Part:BBa K4201020:Design"
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<i>de novo</i> Synthesis was completed by iGem sponsors IDT and Twist Biosciences. | <i>de novo</i> Synthesis was completed by iGem sponsors IDT and Twist Biosciences. | ||
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Revision as of 08:34, 12 October 2022
CrtE_cytoTDS2__T5αOH_RUBY
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6848
Illegal PstI site found at 2087
Illegal PstI site found at 5638
Illegal PstI site found at 8395
Illegal PstI site found at 13603 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6848
Illegal PstI site found at 2087
Illegal PstI site found at 5638
Illegal PstI site found at 8395
Illegal PstI site found at 13603 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6848
Illegal BglII site found at 790
Illegal BglII site found at 1153
Illegal BglII site found at 2021
Illegal BglII site found at 2956
Illegal BglII site found at 4011
Illegal BglII site found at 5324
Illegal BglII site found at 5572
Illegal BamHI site found at 11815
Illegal XhoI site found at 3601
Illegal XhoI site found at 3868 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6848
Illegal PstI site found at 2087
Illegal PstI site found at 5638
Illegal PstI site found at 8395
Illegal PstI site found at 13603 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6848
Illegal PstI site found at 2087
Illegal PstI site found at 5638
Illegal PstI site found at 8395
Illegal PstI site found at 13603
Illegal NgoMIV site found at 1697
Illegal NgoMIV site found at 6997
Illegal NgoMIV site found at 9751
Illegal NgoMIV site found at 10330
Illegal NgoMIV site found at 12043 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 948
Illegal BsaI site found at 1887
Illegal BsaI site found at 2167
Illegal BsaI site found at 3113
Illegal BsaI site found at 5438
Illegal BsaI site found at 5718
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 1873
Illegal BsaI.rc site found at 2153
Illegal BsaI.rc site found at 3100
Illegal BsaI.rc site found at 5424
Illegal BsaI.rc site found at 5704
Illegal BsaI.rc site found at 6651
Design Notes
Part information and design consideration can be found on respective basic parts pages.
Source
CrtE is a GGPP synthase from Pantoea ananatis LMG 20103
RUBY is a reporter gene from the order Caryophyllales.
Gmubi promoters originate from Glycine max.
AtHSP terminators originate from the Arabidopsis thaliana genome.
cytoTDS2 is a taxadiene synthase native to Taxus chinensis var. mairei optimized for use in Glycine max.
T5αOH is a hydroxylase from Taxus baccata that converts taxadiene into taxadiene-5α-ol, a step in the paclitaxel pathway.
de novo Synthesis was completed by iGem sponsors IDT and Twist Biosciences.