Difference between revisions of "Part:BBa K4444015:Design"

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===Design Notes===
 
===Design Notes===
−
n/a
+
<u>Modifications to RELEASE:
 +
 
 +
</u> - TEV cut site is replaced with PhoCl
 +
 
 +
- GFP is replaced with luciferase
 +
 
 +
<u>Components:
 +
 
 +
</u> luciferase, furin cut site, tri-transmembrane domain, PhoCl, and ER retention motif
  
  
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PhoCl: https://www.addgene.org/164037/
 
PhoCl: https://www.addgene.org/164037/
  
−
Lab member's plasmid (William Benman)
+
Luciferase: Lab member's plasmid (William Benman)
  
 
===References===
 
===References===

Latest revision as of 08:15, 12 October 2022


PhoCl Secrete Luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 991
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 604
    Illegal BsaI.rc site found at 1684


Design Notes

Modifications to RELEASE:

- TEV cut site is replaced with PhoCl

- GFP is replaced with luciferase

Components:

luciferase, furin cut site, tri-transmembrane domain, PhoCl, and ER retention motif


Source

RELEASE: https://www.addgene.org/179642/

PhoCl: https://www.addgene.org/164037/

Luciferase: Lab member's plasmid (William Benman)

References

Vlahos, Alexander E., et al. “Protease-Controlled Secretion and Display of Intercellular Signals.” Nature Communications, vol. 13, no. 1, 17 Feb. 2022, p. 912, www.nature.com/articles/s41467-022-28623-y, 10.1038/s41467-022-28623-y.

Zhang, W., Lohman, A., Zhuravlova, Y. et al. Optogenetic control with a photocleavable protein, PhoCl. Nat Methods 14, 391–394 (2017). https://doi.org/10.1038/nmeth.4222